Although the soil heat flux is not a large factor to the heat source on the surface heat balance on the Tibetan Plateau, it is certain one of important factors to keep the heat balance of the earth's surface. On the gradient observational data of the surface soil layer from November 1997 to October 1998 collected by two sets of Automatic Weather Station(AWS) installed in Gaize and Shiquanhe on the western Tibetan Plateau, specially using the soil heat flux underground 2.5 cm and 7.5 cm depths and soil temperature in different depths, the characteristics of temporal variation of the soil heat flux, soil temperature gradient and soil heat storage are analyzed, the composited diurnal cycles are also given by using the soil heat flux, soil temperature gradient and soil heat storage during a whole year. Moreover, on the comparisons of observational soil heat flux to the estimates from soil temperature, the empirical formula is driven in this paper. This study will contributes to understand temporal variations of soil heat transfer and surface heat balance in the western Tibetan Plateau. In addition to, it is helpful to improve the climatology calculation method of soil heat flux.